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PROTEOMICS CENTER
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| Multi-Gel Analysis / Pooled Internal Standard | |
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Multi-gel comparisons may be performed
using different combinations of sample sets. In the example a
control sample (C1)
is labeled with Cy 3, and samples S1,
through Sn are
labeled with Cy 5. A pool of all samples is labeled with Cy 2
and serves as a standard common to each gel. The pooled
standard, the control, and one of the test samples are combined and
run on each gel. Images are generated, and
compared within each two-dimensional gel (using DeCyder DIA image analysis software) and
between gels (using other image analysis software) to
quantitate relative differences among the samples.
Inter-gel analysis is considerably more
time-consuming and inexact than single gel analysis. When possible,
single gel three image pilot analyses are recommended.
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Multi-Gel Analysis / Pooled Internal
Standard Proteomics ExperimentTwo Dimensional Difference Gel Electrophoresis $725/gel Protein Identification (up to 15 gel features) includes $1720/analysis Spot Excision In Situ Gel Digestion MALDI TOF/TOF Mass Spectrometry LC-MS/MS Q-STAR Mass Spectrometry Database Search and Report Creation Removal of High Abundance Human Proteins Fees are for Washington University investigators. Non-WU investigators should contact Jim Malone for pricing. To schedule a meeting to discuss your experimental design call (314) 286-2452 |
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| Proceed to Sample Submission Form for Multi-gel Analysis | |
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Last Updated 10/3/07 |
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