PROTEOMICS 

                         CENTER

 

Single Gel / Three Images
Each protein sample is labeled with either Cy2, Cy3, or Cy5 cyanine dyes, combined, and then resolved on a single two-dimensional gel. A digital image is generated from each Cy dye. In this experimental design, the uncertainties of feature matching are eliminated and manual image analysis is minimized.

The first sample (S1) may be a mixture of standard proteins (provided by the Proteomics Center), a pooled standard generated by combining all of the samples in a multi-gel study (to match the images from this gel with others in the study), or another protein sample for direct comparison to samples S2 and S3. Images are produced and analyzed using DeCyder Differential Analysis software (Amersham BioSciences).

 

Single Gel / Three Image Proteomics Experiment
	Two Dimensional Difference Gel Electrophoresis 				$725/gel
	Protein Identification (up to 15 gel features) includes				$1720/analysis
		Spot Excision
		In Situ Gel Digestion
		MALDI TOF/TOF Mass Spectrometry
		LC-MS/MS Q-STAR Mass Spectrometry
		Database Search and Report Creation
	Removal of High Abundance Human Proteins				$325/sample

Fees are for Washington University investigators. Non-WU investigators should contact Jim Malone for pricing.

To schedule a meeting to discuss your experimental design call (314) 286-2452

 

Proceed to Sample Submission Form

Last Updated 10/3/07