Standard Rate

• Two-Dimensional Gel Electrophoresis of 3 samples (includes)          $750 / 3 sample gel
•     Protein quantification
•     Sample labeling (Cy2, Cy3, and/or Cy5)
•     Mounting gel to glass plate
•     Resolution of individual proteins using 2-D PAGE
•     Digital image generation and storage with back-up
•     Access to image analysis software
•     Gel feature selection guidance
• Protein Identification of up to 15 gel features (includes)                     $1720
•     Robotic Spot Excision
•         Receive picklist from investigator
•         Generate spot map showing selected spots
•         Create robotic (ProPic) picklist from DeCyder picklist
•         Load and align immobilized gel into ProPic robot
•         Excise up to 24 spots and transfer to 96 well plate
•     In Situ Gel Digestion
•         Washing and equilibration of cut gel features 
•         Drying in Speed-Vac
•         In-situ trypsinization (alternate protease available as custom application)
•         Spotting digested sample aliquots to MALDI plate
•         Re-extraction of peptides from gel plugs
•         Preparation of samples for Q-STAR analysis
•     MALDI TOF/TOF Mass Spectrometry
•         MALDI-TOF/TOF calibration (observed vs theoretical at 10-20 ppm) and sensitivity check (~50 fmol)
•         Optimized method preparation
•         Automated MS and MS/MS spectral acquisition
•         Database searching
•         Archiving all MS and MS/MS files to storage with back-up
•     LC-MS/MS Q-STAR Analysis
•         LC-MS/MS calibration and "benchmarking"
•         Optimized method preparation
•         Automated sample injection and LC separation
•         MS and MS/MS spectral acquisition
•         Database searching
•         Archiving all MS and MS/MS files to storage with backup
•     Database Searching with Spectral Interpretation and Reporting
•         Load MS and MS/MS data into database search engine
•         Build conceptually translated databases as needed
•         Acquire protein identification list and putative peptide sequences
•         Check peptide sequences with fragmentation spectra
•         Review findings with investigators
• Removal of 6 High Abundance Human Proteins (optional)                    $325
•     Preparation of human serum or human cerebrospinal fluid for affinity chromatography
•     Depletion of 6 most abundant proteins using a chromatographic multi-affinity method
•     Sample concentration and desalting for two-dimensional gel electrophoresis